发明名称 |
smFRET WITH MEMBRANE PROTEINS |
摘要 |
This disclosure is directed to methods of conducting dynamic single-molecule fluorescence studies such as sm-FRET on a membrane protein which permits observation and quantification of conformational dynamics of a membrane protein. Also disclosed herein are mutant membrane proteins in which one or more mutations have been introduced for affixing a fluorophore, as well as reagents and kits containing such mutant membrane proteins for conducting dynamic single-molecule fluorescence studies. The methods and compositions disclosed herein can be used in screening for compounds that enhance or reduce the activity of a membrane protein, useful for treating diseases associated with the malfunction of the membrane protein or alterations in membrane protein conformation.
|
申请公布号 |
US2013122525(A1) |
申请公布日期 |
2013.05.16 |
申请号 |
US201113697469 |
申请日期 |
2011.05.13 |
申请人 |
BLANCHARD SCOTT;WEINSTEIN HAREL;JAVITCH JONATHAN;CORNELL UNIVERSITY |
发明人 |
BLANCHARD SCOTT;WEINSTEIN HAREL;JAVITCH JONATHAN |
分类号 |
G01N21/64;C07K14/00 |
主分类号 |
G01N21/64 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|