发明名称 |
ENZYME-ASSISTED SPATIAL DECORATION OF BIOMATERIALS |
摘要 |
A method of producing a hydrogel compromising a spatially-controlled, three- dimensional distribution of one or more bioactive signals is provided. The method compromises illuminating the hydrogel, wherein the hydrogel compromises a polymer bound to a peptide comprising a photolabile protected amino acid, wherein at least one portion of the hydrogel is illuminated to deprotect the protected amino acid, thereby converting the protected amino acid to a deprotected amino acid. Preferably, the deprotected amino acid is a substrate for an enzyme in at least one portion of the hydrogel. The method further comprises the step of contacting the hydrogel with the enzyme and a peptide comprising a bioactive signal, wherein the enzyme can form a bond between the substrate and the peptide comprising the bioactive signal, thereby producing a hydrogel compromising a plurality of bioactive signals occupying three dimensions of the hydrogel within at least one portion of the hydrogel subjected to illumination. |
申请公布号 |
WO2013071126(A1) |
申请公布日期 |
2013.05.16 |
申请号 |
WO2012US64475 |
申请日期 |
2012.11.09 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA;SEGURA, TATIANA;GRIFFIN, DONALD |
发明人 |
SEGURA, TATIANA;GRIFFIN, DONALD |
分类号 |
A61L27/52;A61L27/54;A61L27/56;C12N11/04 |
主分类号 |
A61L27/52 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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