摘要 |
The invention relates to methods of generating one or more copies of a target polynucleotide molecule, that contains a repeat of identical nucleotides. These methods comprise the steps of : using a primer which hybridizes with a part of a homonucleotide stretch in the target molecule, but is not completely complementary with the homonucleotide stretch in the target molecule. Within the portion of the oligonucleotide primer hybridizing to the homonucleotide stretch in the target molecule, at least one mismatch is incorporated, compared to the homonucleotide stretch in the target molecule. The amplified molecule is more easily sequencable than the sequence which contains the entire repeat of identical nucleotides. |