CONSTRUCT FOR REGULATING FERTILITY OF PLANT POLLENS AND USAGE THEREOF

摘要

Disclosed is a method for creating a male sterility line of a plant, comprising: using two different specific expression promoters of the late development stage of plant pollens to drive two nucleotide fragments, namely the N terminus and C terminus of the ribonuclease gene Barnase of Bacillus, to express in the plant, respectively. As both the small peptides encoded by the N terminus and C terminus of Barnase have no ribonuclease activity, when driven to express in the same cell by the two pollen-specific promoters of which the expression periods overlap, the peptides can recover partially natural ribonuclease activity, leading to male sterility of the plant.