BEAD EMULSION NUCLEIC ACID AMPLIFICATION

摘要

PROBLEM TO BE SOLVED: To provide methods for amplifying nucleic acid templates from low copy number to quantities amenable for sequencing on a solid support such as a bead.

SOLUTION: The method for amplifying the nucleic acids includes the steps of: forming a water-in-oil emulsion to create a plurality of aqueous microreactors wherein a nucleic acid template, a bead, and an amplification reaction solution are emulsified; amplifying the nucleic acids in the microreactors to form amplified copies of the nucleic acids; and binding the amplified copies to the beads in the microreactors.

COPYRIGHT: (C)2013,JPO&INPIT