发明名称 |
PRODUCTION CELL LINE |
摘要 |
The invention refers to a method of producing a recombinant polypeptide of interest (POI) in a cell culture, comprising genetically engineering a eukaryotic cell line—to specifically cause prolongation of the G2+M cell cycle phase in a pre-culture phase, and—to produce the POI in a producing phase following the pre-culture phase, a high producer cell line and cell culture as well as a method of increasing the yield of a recombinant POI production in a cell culture. |
申请公布号 |
EP2591121(A1) |
申请公布日期 |
2013.05.15 |
申请号 |
EP20110728885 |
申请日期 |
2011.07.04 |
申请人 |
UNIVERSITAET FUER BODENKULTUR WIEN |
发明人 |
MATTANOVICH, DIETHARD;DRAGOSITS, MARTIN;GASSER, BRIGITTE;MAURER, MICHAEL;SAUER, MICHAEL |
分类号 |
C12P21/02;C12N1/15;C12N5/10;C12N15/67 |
主分类号 |
C12P21/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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