ELECTRODE SYSTEM FOR MEASURING AN ANALYTE CONCENTRATION UNDER IN-VIVO CONDITIONS

摘要

The electrode system comprises a counter electrode (2) with an electric conductor, a working electrode (1) with an electric conductor on which an enzyme layer is arranged that consists of immobilized enzyme molecules for catalytic transformation of analytes, and a diffusion brake, which slows down the diffusion of the analytes from body fluid surrounding the electrode system, to enzyme molecules. The enzyme layer is formed in the form of several fields that are arranged to each other on the conductor of the working electrode in an interval. The electrode system comprises a counter electrode (2) with an electric conductor, a working electrode (1) with an electric conductor on which an enzyme layer is arranged that consists of immobilized enzyme molecules for catalytic transformation of analytes, and a diffusion brake, which slows down the diffusion of the analytes from body fluid surrounding the electrode system, to enzyme molecules. The enzyme layer is formed in the form of several fields that are arranged to each other on the conductor of the working electrode in an interval. The two of the fields of the enzyme layer of 5 mm are removed from one another. An interval between adjoining fields of the enzyme layer is 0.3 mm. The fields of the enzyme layer of 2 mm extend itself in two directions vertical to each other. The diffusion brake is formed as a layer covering the enzyme layer. The enzyme cooperates with a catalytic redox mediator that reduces or prevents an oxygen dependence of the catalytic conversion of the analytes. The catalytic redox mediator transfers hydrogen peroxide and causes a direct electron transfer. The electrode system is bent without breaking around 90[deg] . The working electrode comprises a spacer that is arranged by the conductor over the enzyme layer. The spacer covers the working electrode and the counter electrode as solid layer. The electric conductors of the working electrode and the counter electrode are arranged on a substrate (4). The conductor of the working electrode has narrow positions between the enzyme layer fields and the conductor of the counter electrode has contour following the progress of the conductor of the working electrode. An independent claim is included for a sensor with electrode system.