发明名称 |
Methods and compositions for generating and amplifying DNA libraries for sensitive detection and analysis of DNA methylation |
摘要 |
The present invention regards a variety of methods and compositions for obtaining epigenetic information, such as DNA methylation patterns, through the preparation, amplification and analysis of Methylome libraries. In particular, the method employs preparation of a DNA molecule by digesting the DNA molecule with at least one methylation-sensitive restriction enzyme; incorporating a nucleic acid molecule into at least some of the digested DNA molecules by either (1) incorporating at least one primer from a plurality of primers that have a 5' constant sequence and a 3' variable sequence, wherein the primers are substantially non-self-complementary and substantially non-complementary to other primers in the plurality; or (2) incorporating an oligonucleotide having an inverted repeat and a loop under conditions wherein the oligonucleotide becomes blunt-end ligated to one strand of the digested DNA molecule, followed by polymerization from a 3' hydroxyl group present in a nick in the oligonucleotide-linked molecule; and amplifying one or more of the DNA molecules.
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申请公布号 |
US8440404(B2) |
申请公布日期 |
2013.05.14 |
申请号 |
US20050071864 |
申请日期 |
2005.03.03 |
申请人 |
MAKAROV VLADIMIR L.;KAMBEROV EMMANUEL;SUN TONG;PINTER JONATHON H.;TARRIER BRENDAN J.;BRUENING ERIC E.;KURIHARA TAKAO;TESMER TIM;M'MWIRICHIA JOSEPH;RUBICON GENOMICS |
发明人 |
MAKAROV VLADIMIR L.;KAMBEROV EMMANUEL;SUN TONG;PINTER JONATHON H.;TARRIER BRENDAN J.;BRUENING ERIC E.;KURIHARA TAKAO;TESMER TIM;M'MWIRICHIA JOSEPH |
分类号 |
C12Q1/68;C12N15/10;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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