MEDIUM FOR PRODUCTION OF SEPIAPTERIN WITH RICE PROTEIN AND METHOD FOR PRODUCTION OF SEPIAPTERIN THEREFROM

摘要

PURPOSE: A medium for producing sepiapterin containing a rice protein and a method for producing sepiapterin using the same are provided to obtain high yield and high productivity sepiapterin using rice protein hydrolysate as a nitrogen source. CONSTITUTION: A medium for producing sepiapterin contains a rice protein or a rice protein hydrolysate. The rice protein or the rice protein hydrolysate is added as a nitrogen source. A method for producing the rice protein comprises: a step of pulverizing rice; a step of adding a lipid soluble material-extracting solvent and suspending and removing lipid; a step of adding an alkaline solution to the rice and adjusting to pH 9.0-9.5; a step of centrifuging to remove insoluble materials and collecting a primary supernatant; a step of adding an acidic solution to the primary supernatant and adjusting to pH 4.0-4.5; a step of centrifuging, removing the supernatant, and collecting a primary precipitate; a step of adding distilled water to the primary precipitate and freeze-drying. A method for producing sepiapterin comprises a step of culturing a transformed microorganism in the medium. The microorganism is E.coli. [Reference numerals] (AA) Rice; (BB) Pulverize(120 mesh); (CC) Extract lipid(n-hexane, 3 times); (DD) Dry(12 hr); (EE) Extract protein(pH 9.5); (FF,II,LL) Centrifuge(12,000rpm, 10min); (GG) Supernatant; (HH) Precipitate protein(pH 4.5); (JJ) Precipitate; (KK) Wash(pH 4.5); (MM) Neutralize(pH 7.0); (NN) Freeze-dry; (OO) Protein powder