| 摘要 |
The purpose of the present invention is to provide: a novel method for detecting a target nucleic acid; and a kit for use in the method. In the detection method according to the present invention, a fluorescently labeled primer/probe and a quencher-labeled probe, which have complementarity to each other, are so designed as to have different melting temperatures (Tm) from each other so that the fluorescently labeled primer can anneal preferentially to the target nucleic acid. The detection method is so designed that the fluorescently labeled primer/probe that is not bound to the target nucleic acid is bound to the quenching primer so as to emit no fluorescence. The method enables the detection of the target nucleic acid in a simpler manner, at lower cost, and without requiring the use of any technique or device. |
