PROCESS FOR PRODUCTION OF CLAVULANIC ACID

摘要

The present invention belongs to the field of pharmaceutical industry and specifies the process for preparing clavulanic acid by using a microorganism, comprising the steps of providing a microorganism being capable of producing clavulanic acid and which produces d-(L-a-aminoadipyl)-L- cysteinyl-D-valine synthetase (ACVS), selectively reducing or abolishing ACVS activity by modifying or treating said microorganism, cultivating it and isolating clavulanic acid. Furthermore, the present invention refers to a process for preparing clavulanic acid by using said modified microorganism. Further, the present invention relates to a recombinant plasmid comprising a modified pcbAB gene, to a recombinant microorganism comprising said recombinant plasmid, and to the use of said recombinant plasmid for modifying a microorganism. Finally, the present invention also refers to a microorganism that is modified or treated by selectively reducing or abolishing activity of ACVS, as well as to the use of said microorganism for the production of clavulanic acid.