METHOD FOR CONSTRUCTING METHYLATED HIGH-THROUGHPUT SEQUENCING LIBRARY FOR WHOLE GENOME AND USE THEREOF

摘要

Provided are a method for constructing a methylated high-throughput sequencing library for a whole genome and the use thereof. The method for constructing a methylated high-throughput sequencing library for a whole genome comprises: enzyme-digesting genome DNA with Msp I and a second restriction endonuclease; end-repairing the DNA fragments; adding base A at the 3' end of the DNA fragments that have been end-repaired; linking the DNA fragments with cohesive end A with a methylated linker; selecting fragments among the linking products with a methylated linker so as to obtain a target fragment; treating the target fragment with bisulfate so as to transform non-methylated cytosine in the target fragment to uracil; PCR amplifying the transformed target fragment; and isolating and purifying the amplification products. The amplification products constitute the methylated high-throughput sequencing library for the whole genome. The whole genome methylated high-throughput sequencing library of the genome DNA sample can be conveniently and effectively constructed using the method for constructing a methylated high-throughput sequencing library for a whole genome and the use thereof of the present invention.