SYNTHETIC OLIGONUCLEOTIDE PRIMERS-PROBE AND METHOD TO DETECT GENOMES OF 1ST, 4TH, 16TH SEROTYPES OF BLUETONGUE DISEASE VIRUS BY METHOD OF RT-PCR IN REAL-TIME MODE

摘要

FIELD: biotechnologies.SUBSTANCE: synthetic oligonucleotide primers and probes are disclosed to detect genomes of 1st, 4th and 16th serotypes of the bluetongue disease virus. Also the method is described to detect genomes of 1st, 4th and 16th serotypes of the bluetongue disease virus using such synthetic oligonucleotide primers and probes.EFFECT: method makes it possible to perform determination of a serotype of the bluetongue disease virus with the help of PCR with a stage of reverse transcription in real time mode, and also to increase specificity and sensitivity, to reduce time to perform work aimed at determination of a serotype of the bluetongue disease virus in samples of an investigated biological material.4 cl, 4 tbl