Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor.
申请公布号
US2013115681(A1)
申请公布日期
2013.05.09
申请号
US201313736529
申请日期
2013.01.08
申请人
NEW ENGLAND BIOLABS, INC.;NEW ENGLAND BIOLABS, INC.