FUSION PROTEIN OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS AS PRRS VACCINE

摘要

A fusion protein of porcine reproductive and respiratory syndrome virus (PRRSV) is disclosed. The fusion protein comprises: a) a Pseudomonas exotoxin A (PE) peptide comprising a binding domain and a translocation domain, located at the N-terminus of the fusion protein; b) a fusion peptide of American strain PRRSV ORF6-2~26 (SEQ ID NO: 13) and ORF5-31~63 (SEQ ID NO: 12), or a fusion peptide of European strain PRRSV ORF6-M1~I28 (SEQ10 NO: 14) and ORF5-F31~A64 (SEQ ID NO: 15); and c) a carboxyl terminal domain comprising the amino acid sequence of KDEL-KDEL-KDEL located at the C-terminus of the fusion protein, in which the capital letters 'KDEL' represent single letter amino acid codes, wherein the ORF5-31~63 located between the ORF6-2~26 and the carboxyl domain, and the ORF6-F31~A64 is located between the ORF6-M1~I28 and the carboxyl domain.