MEDIUM COMPOSITION FOR ACCELERATING GROWTH OF PHOTOSYNTHETIC MICROORGANISM AND ANIMAL CELL AND THE MANUFACTURING METHOD

摘要

PURPOSE: A culture composition for promoting photosynthetic microorganism and animal cell growth and a manufacturing method thereof are provided to accelerate the production of photosynthetic microorganisms and promote growth by promoting the animal cells and reducing the stress in the cells. CONSTITUTION: A culture composition for promoting photosynthetic microorganism and animal cell growth includes a mutant strain of Rhodobacter sphaeroides, MBTLJ- 8(accession number: KACC91572P) as an active ingredient. The generation process of the mutant strain comprises the following steps: adding N-Methyl-N'-nitro-Nnitrosoguanidine to a rhodobacter sphaeroides culture medium; and culturing the same at 15 Watts for 48 hours. The culture composition for promoting photosynthetic microorganism and animal cell growth includes a mutant strain of Rhodobacter sphaeroides, photosynthetic activating(or alternative) compound as an active ingredient. A manufacturing method of culture composition for promoting photosynthetic microorganism and animal cell growth comprises the following steps: a first step of manufacturing culture medium 1 by shake culturing the Rhodobacter sphaeroides at 30°C for 48 hours; a second step of manufacturing culture medium 2 by mainly culturing the culture medium 1 at 30°C for 48 hours; and a third step of manufacturing a culture composition containing Rhodobacter sphaeroides mutant strain, MBTLJ-8 by cultivating at 15 Watts for 48 hours after adding N-Methyl-N'-nitro-N-nitrosoguanidine to the culture medium 2. [Reference numerals] (AA) Inoculate Rhodobacter sphaeroides into 5ml of sistrom's minimal medium and shake culture at 30°C and 140rpm for 24 hours; (BB) Add 1ml of the culture medium into 100ml of the sistrom's minimal medium and main culture at 30°C and 150rpm for 24 hours under the dark state; (CC) Add 1ml of the culture medium into 100ml of the sistrom's minimal medium and main culture at 30°C and 150rpm for 24 hours under the light state; (DD) Add 1ml of the culture medium into 100ml of the sistrom's minimal medium, main culture at 30°C and 150rpm for 12 hours under the 15W light state and for 12 hours under the dark state; (EE) Arrange the cultured cells and medium in a tube, centrifuge at 4°C and 5000rpm for 30 minutes, and collect precipitated cells; (FF) Adjust volume by adding sterilized water into the cells which are collected to be as much as 1/20 of the culture medium and sonicate using an ultra sonicator under a refrigerating state for 30 minutes based on the condition of 20 seconds pulse and 10 seconds recess; (GG) Name a solution obtained by filtering supernatant, which is centrifuged at 4°C and 13000rpm for 30 minutes, using 0.2um filter as PACs