Non-natural amino acid substituted polypeptides

摘要

The invention provides compositions and methods of identifying, modifying and producing modified target molecules, including therapeutic molecules by modification with non-natural amino acids. Certain aspects of the invention include methods of adding a chemical moiety to a target molecule, and the compositions resulting therefrom. Certain aspects of the inventions also relate to kits for identifying, modifying and producing modified target molecules described herein. -- Vehicle 3x/wk 600 -- BS IOU 3x/week 10K PEG 10U 1x/wk 40 -- BS 1OU lxwk p.001 o 20 25 30 35 40 45 50 55 60 65 day post implant -'-- Vehicle 600 - BS 1OU 3X/wk -- 2OK PEG 3U 1X/wk -B- 5104 IX/wk 0 -0.020 25 30 35 40 45 50 55 60 65 Day post implant