Phased small RNAs

摘要

Abstract This invention discloses recombinant DNA constructs encoding phased small RNAs useful in regulating expression of one or more genes of interest. Also disclosed by this invention are transgenic plant cells, plants, and seeds containing a recombinant DNA construct of this invention WO 2008/027592 PCT/US2007/019283 60M -5Fend position n of sIR NA m1 P2 P3 ArPSI Ph'l pr eI 7P1S 4',P9p1 1 00bp I- J _ 0s06g2l90C TAr~IAAA 1 kbp SEQ 10 SEQ ID SEQ ID B22-3 2927 SSj OB 53.1 20 21,9 47 . 3'P1 P2 P3 P4 P50 1169 P7m PS PIKE P2x PIN' N. PS P6 P7 pan 11.0 23.9 3315.1 1427.2 1586.3 35084.5 120.2 SEQ ID SEQ ID SEQ 10 SEQ ID NO. 3 NO.4 NO. 5 NO. 6 C Rice Maize Soy Arabidopsis Seedling Grain Lr~zf Root Kernel Mature Leaf Young Leaf L~eaf 24-, -- 21- . D CoI-0 ddl 7 dcI4-1 21- siR-P5 21 -'miRl 73 21 ta-siR25 5 SS RNA/tRNA