| 摘要 |
<P>PROBLEM TO BE SOLVED: To rapidly, highly precisely, highly sensitively, and conveniently detect and determine a small number of microbes contained in a large amount of sample. <P>SOLUTION: A sample container 102 has a two-layer membrane filter 101 on the bottom, which includes: a first layer 116 being an upper layer serving as a hydrophilic membrane filter (with an opening diameter of 0.05-0.65 μm) and; a hydrophobic membrane filter 117 as an second layer that can filter an aqueous solution without using a wetting agent by means of a formed negative pressure. Using the sample container, a large amount of aqueous sample solution is filtered by means of the negative pressure formed by a suction portion 105 to capture microbes in the aqueous sample solution by the hydrophilic membrane filter. After the negative pressure is restored to normal pressure, a microbial dissolution solution is added to retain the microbial dissolution solution for a given time on the hydrophobic membrane filter. Then, the microbial dissolution solution is dispensed to a reaction container 113 containing a luminescent sample 114, and luminescence is detected to detect the microbes. <P>COPYRIGHT: (C)2013,JPO&INPIT |
