Method for improving the stability of purified Factor VIII after reconstitution

摘要

CSL BEHRING GMBH 2011_M008_A186 HAU/kk 5 Abstract The present invention relates to a method for increasing the stability of a Factor VIII molecule after purification, lyophilization and reconstitution, comprising preventing proteolytic cleavage of the Factor VIII molecule into a first fragment comprising 10 essentially the Al domain and the A2 domain and a second fragment comprising essentially the A3 domain, the C1 domain and the C2 domain throughout manufacturing of the Factor VIII molecule. The invention further pertains to a method for improving the bioavailability of Factor VIII after intravenous and non intravenous injection.