PROCESS OF DIAGNOSTIC, PROGNOSTIC AND THERAPEUTIC MONITORING OF SOLID TUMORS

摘要

The present invention relates to a process of diagnostic, prognostic and therapeutic monitoring of solid tumors. An improved recovery process of single viable cells from a solid tumor giving both a higher yield of the number of viable cells per gram of tumor and an increased percentage of viability, suitable for further cell surface component analysis useful in diagnostic, prognostic or therapeutic response assessment. Said process involves a step using a non enzymatic dissociation buffer and a step involving enzymatic tissue dissociation followed by a cell purification step with dual density Ficoll. The invention also relates to new biological markers of tumor.