METHODS OF DETECTING BRAF MUTATIONS IN CANCER

摘要

The disclosure relates to an allele-specific PCR assay for BRAF V600E mutation. The forward primer comprises one of the following sequences: AGGTGATTTTGGTCTAGCTACAGA; G GTG ATTTTG GTCTAG CTACAG A; AGGTGATTTTGGTCTAGCTACCGA; or G GTG ATTTTG GTCTAG CTACCG A; and the reverse primer comprises GTA ACTC AG C AG C ATCTC AG G G. The assay is suitable for use in diagnosis of hairy cell leukemia.