Microorganisms for the production of 1,4-butanediol and related methods

摘要

The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO. glucoseex Glycolysis ED pathway 0.20 0.270.19 PYRex PEP 0 -0.86±0.24 0 PYR 4-HBO 1.15±0.01[ -> ACEex 4-HBCoAO 0.22t0.09) OAcCoA (0.27±0.08) (6000.85t0.08 0AA ITF/ICT 0.57t0.01 0.00±0.00 a-KG MALO -0.00+0.00 0 0 4HB/GBL GOX 0.40+0.08 0.22±0.01 SSA 0.20t0.00 FUM 0.12±0.08) 0-0300.00)into BDO pathway OFU 020.80.10±0.00 O0,0(0.10±0.08 BDoey SUCr SucCoA 400.00 ECKh-138 ECKh-422 350.00A BO Ace 180.0- D 7 S300.00-~16. - R- 140.0 - 5.0 _-, Pyr E100 - 120.0 200.00 4HB 100.0 4HB S80.0150.000.0 ce 0O her0. BO 40.0 50.00-20.0 L Other 0.09 Other 0.00 10 20 30 40 50 60 0 20 40 60 80 100 120 140 Time since induction (hours) Time since induction (hours) FIG. 47A FIG. 47B