发明名称 |
Glycoprotein composition from engineered galactose assimilation pathway in Pichia pastoris |
摘要 |
Lower eukaryotic cells such as Pichia pastoris that normally cannot use galactose as a carbon source but which have been genetically engineered according to the methods herein to use galactose as a sole source of carbon are described. The cells are genetically engineered to express several of the enzymes comprising the Leloir pathway. In particular, the cells are genetically engineered to express a galactokinase, a UDP-galactose-C4-epimerase, and a galactose-1-phosphate uridyltransferase, and optionally a galactose permease. Glycoproteins are provided wherein the ratio of G0:G1/G2 glycoforms therein is less than 2:1. |
申请公布号 |
EP2546347(A3) |
申请公布日期 |
2013.05.01 |
申请号 |
EP20120188235 |
申请日期 |
2010.02.24 |
申请人 |
MERCK SHARP & DOHME CORP. |
发明人 |
DAVIDSON, ROBERT C.;BOBROWICZ, PIOTR;ZHA, DONGXING |
分类号 |
C12N15/81;C07K16/18;C07K16/28;C07K16/30;C12N1/16;C12N9/12;C12N9/90;C12N15/54;C12N15/61;C12P21/00 |
主分类号 |
C12N15/81 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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