| 摘要 |
The present invention relates to a method for focusing an image of a slide (1, 1') provided with one or more pits (10) in a fluorescent test for autoimmunity. The method is implemented through an optical microscope (30) provided with at least two lenses (41) to acquire images at two different magnifications and a movable table on which is placed the slide. According to the method are provided the operations of:
- Selection of a lens at a first magnification (B.I.) and determination of a first focus distance (Z-foc-BI) at said first magnification (B.I.) for at least one pit (10, 10') of the slide (1, 1'), said operation comprising the acquisition and focus analysis at different distances (Z) of acquired images at a point predetermined of the matrix of the slide located outside of the pit and substantially at the same level of the pit under examination;
- Subdivision of the pit under examination in a plurality of points (200', 200) defining an acquisition path of images;
- Selection of a second magnification (A.I.) greater than the first magnification (B.I.);
- For each point (200', 200) determination of a punctual focus distance (Z-foc-punt) at said second magnification (A.I.) through the acquisition at different distances (Z) and the focus analysis of images acquired in the point (200', 200) with a gain value (G) of the camera and aperture time (T) such that said images all have a predetermined value of constant light intensity (I);
- Determination of a second focus distance (Z-foc-AI) of the pit at said second magnification (A.I.) under examination as a function of the calculated punctual focus distances (Z-foc-punt);
- and wherein, furthermore, the first focus distance (Z-foc-BI) at low magnification is used as starting level (Z) for the determination of the punctual focus distance (Z-foc-punt) at a point of the path (200', 200). |
