| 摘要 |
THE PRESENT INVENTION RELATES TO A METHOD FOR THE LARGE SCALE IN VIVO SYNTHESIS OF SIALYLATED OLIGOSACCHARIDES, CULTURING A MICROORGANISM IN A CULTURE MEDIUM, OPTIONALLY COMPRISING AN EXOGENOUS PRECURSOR SUCH AS LACTOSE, WHEREIN SAID MICROORGANISM COMPRISES HETEROLOGOUS GENES ENCODING A CMP-NEU5AC SYNTHETASE, A SIALIC ACID SYNTHASE, A GLCNAC-6-PHOSPHATE 2 EPIMERASE AND A SIALYTRANSFERASE, AND WHEREIN THE ENDOGENOUS GENES CODING FOR SIALIC ACID ALDOLASE (NANA) AND FOR MANNAC KINASE (NANK) HAVE BEEN DELETED OR INACTIVATED. THE INVENTION ALSO RELATES TO THESE MICROORGANISMS WHICH ARE CAPABLE OF PRODUCING INTERNALLY ACTIVATED SIALIC ACID. |
