Bacterial cells, optimized nucleotide sequences and methods for improved expression of recombinant Clostridium difficile toxin B

摘要

In some embodiments, the present invention provides isolated nucleotide sequences that encode Clostridium difficile toxin B, wherein the isolated nucleotide sequences have been optimized for improved expression of the toxin B in a bacterial cell. Other embodiments of the present invention pertain to methods of expressing recombinant Clostridium difficile toxin B in a bacterial cell from the isolated nucleotide sequences of the present invention. In other embodiments, the present invention pertains to bacterial cells that comprise the isolated nucleotide sequences of the present invention. In further embodiments, the invention pertains to isolated peptides of recombinant Clostridium difficile toxin B that were derived from the isolated nucleotide sequences of the present invention.