METHODS OF AMPLIFYING AND SEQUENCING NUCLEIC ACIDS

摘要

A method for sequencing nucleic acids. The method includes fragmenting large template nucleic acid molecules to generate a plurality of fragmented nucleic acids; attaching one strand of a plurality of the fragmented nucleic acids individually to beads to generate single stranded nucleic acids attached individually to beads; delivering a population of the single stranded fragmented nucleic acids attached individually to beads to an array of at least 10,000 reaction chambers on a planar surface, wherein a plurality of the reaction chambers comprise no more than one bead with one single stranded fragmented nucleic acid; amplifying the single stranded nucleic acids in the reaction chambers; and, performing a sequencing reaction simultaneously on a plurality of the reaction chambers.