METHOD AND USES FOR BOMBYX MORI SILK FIBROIN HEAVY CHAIN GENE MUTATION SEQUENCE AND MUTANT

摘要

Provided is a method for Bombyx mori silk fibroin heavy chain gene mutation, specifically comprising causing the mRNA of a coded zinc finger nuclease sequence to act upon a target point such as positions 1325-1362 of Bombyx mori silk fibroin heavy chain gene as expressed in SEQ ID NO:1, so as to obtain a series of Bombyx mori silk fibroin heavy chain mutant genes. Also provided is a mutation sequence prepared according to said method. Also provided are uses of said mutation sequence in the preparation of sericin and foreign proteins. Also provided is a Bombyx mori mutant comprising the described mutation sequence as a novel Bombyx mori silk gland bioreactor, the rear silk gland of said mutant being severely degraded and the cocoon layer whereof including only sericin synthesized and excreted by the middle silk gland, whereby the expression volume and purity of foreign transgenes in the rear silk gland of Bombyx mori can be greatly increased and large-scale production of sericin can be achieved.