发明名称 |
METHODS OF CELL CULTURE FOR ADOPTIVE CELL THERAPY |
摘要 |
An improved method of culturing cells for cell therapy applications that includes growing desired cells in the presence of antigen-presenting cells and/or feeder cells and with medium volume to surface area ratio of up to 1 ml/cm2 if the growth surface is not comprised of gas permeable material and up to 2 ml/cm2 if the growth surface is comprised of gas permeable material. The desired cells are at a surface density of less than 0.5×106 cells/cm2 at the onset of a production cycle, and the surface density of the desired cells plus the surface density of the antigen presenting cells and/or feeder cells are at least about 1.25×105 cells/cm2.
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申请公布号 |
US2013102075(A1) |
申请公布日期 |
2013.04.25 |
申请号 |
US201213475700 |
申请日期 |
2012.05.18 |
申请人 |
VERA JUAN F.;ROONEY CLIONA M.;LEEN ANN M.;WILSON JOHN R. |
发明人 |
VERA JUAN F.;ROONEY CLIONA M.;LEEN ANN M.;WILSON JOHN R. |
分类号 |
C12M1/04 |
主分类号 |
C12M1/04 |
代理机构 |
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代理人 |
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地址 |
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