| 摘要 |
<p>The present invention relates to a method for diagnosing a Transmissible Spongiform Encephalopathy (TSE) in a subject, wherein said subject is not a caprinae and said TSE is not scrapie, preferably said TSE being bovine spongiform encephalopathy (BSE) or Creutzfeldt- Jakob disease (CJD), and wherein said method comprises the steps of a) mixing a biological sample from said subject with a caprinae PrPc protein isoform having a glutamine amino acid residue at its position 171, derivative or fragment thereof to make a reaction mixture; b) incubating said reaction mixture in conditions allowing the amplification of the PrPSc protein present in the biological sample; c) disrupting the reaction mixture; d) repeating steps (b) and (c) one or more times; and e) detecting the PrPSc protein in the amplified reaction mixture; and the associated kit.</p> |
