发明名称 |
Recombinant expression vector for animal cell |
摘要 |
The present invention relates to a recombinant expression vector for an animal cell containing a dihydrofolate reductase (DHFR) coding nucleotide sequence operatively linked to a DHFR promoter, to an animal cell line transformed by the vector, and to a method for preparing a target protein using the same. As compared with existing animal cell expression vectors, the vector of the present invention enables an effective screening of a cell line clone in which foreign genes are amplified together with DHFR genes even at a much lower methotrexate concentration. The present invention exhibits excellent effects in cell line preparation as high-productivity cell lines can be ensured in a short time through the use of a lower concentration of methotrexate in the process of protein production cell line establishment. |
申请公布号 |
US8426570(B2) |
申请公布日期 |
2013.04.23 |
申请号 |
US200913002814 |
申请日期 |
2009.07.07 |
申请人 |
KIM KEUN SOO;JU HYE KYUNG;JEON CHOON JU;KIM JAE SEOB;APROGEN INC. |
发明人 |
KIM KEUN SOO;JU HYE KYUNG;JEON CHOON JU;KIM JAE SEOB |
分类号 |
C07H21/02;C07H21/04;C12N5/00;C12N5/02;C12N15/00 |
主分类号 |
C07H21/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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