BIO-MIMETIC ULTRATHIN HYDROGEL COATINGS FOR PANCREATIC ISLET TRANSPLANTATION

摘要

The present disclosure provides multifunctional cytoprotective materials applied to coat living cells or aggregates of cells such as, but not limited to, pancreatic islets. The coating utilizes hydrogen-bonded interactions of a natural polyphenol (tannic acid) with poly(N-vinylpyrrolidone) deposited on the cell aggregate surface via non-ionic layer-by-layer assembly. The coating is conformal over the surface of such as mammalian islets. The coated islets maintain their viability and cell functionality for at least 96 hours in vitro. The coating demonstrates immunomodu latory cytoprotective properties suppressing pro- inflammatory cytokine synthesis in stimulated bone marrow-derived macrophages and diabetogenic B DC-2.5 T cells. The coating material combines high chemical stability under physiologically relevant conditions with capability of suppressing cytokine synthesis, crucial parameters for prolonged islet integrity, viability, and function in vivo.