发明名称 |
Transposon-based targeting system |
摘要 |
The present invention relates to a targeting system comprising, preferably as distinct components (a) a transposon which is devoid of polynucleotide encoding a functional transposase comprising (aa) a polynucleotide of interest and; (ab) a DNA sequence specifically recognized by a DNA binding domain; and (ba) a fusion protein comprising (i) said DNA binding domain; or (ii) a (poly)peptide binding domain binding to a (poly)peptide comprising said DNA binding domain; and (iii) a DNA targeting domain; or (iv) a (poly)peptide binding domain that binds to a cellular or engineered (poly)peptide that comprises a DNA targeting domain; or (bb) a polynucleotide encoding the fusion protein of (ba); and (ca) a transposase or a fragment or derivative thereof having transposase function; or (cb) a polynucleotide encoding the transposase or fragment or derivative thereof having transposase function of (ca). |
申请公布号 |
US8420386(B2) |
申请公布日期 |
2013.04.16 |
申请号 |
US20040544971 |
申请日期 |
2004.02.10 |
申请人 |
IVICS ZOLTAN;IZSVAK ZSUZSANNA;MAX-DELBRUCK-CENTRUM FUR MOLEKULARE MEDIZIN (MDC) |
发明人 |
IVICS ZOLTAN;IZSVAK ZSUZSANNA |
分类号 |
C12N15/00;A61K48/00;C12N5/10;C12N15/64;C12N15/87;C12N15/90 |
主分类号 |
C12N15/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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