发明名称 |
Methods of unidirectional, site-specific integration into a genome, compositions and kits for practicing the same |
摘要 |
The subject invention provides a unidirectional site-specific integration system for integrating a nucleic acid into the genome of a target cell. The provided systems include a (1) a mutant, unidirectional site specific integrase, which can be provided by an integrase vector encoding the mutant integrase and (2) a targeting vector that includes: (a) a nucleic acid to be integrated; and (b) a vector attachment site, where the targeting vector attachment site serves as a substrate for the mutant, unidirectional site-specific integrase. In using the subject systems for site-specific integration, the targeting vector and integrase are introduced into the target cell and the cell is maintained under conditions sufficient to provide for site-specific integration of the nucleic acid into the target cell genome via a recombination event mediated by the site-specific integrase. Also provided are kits that include the subject systems. The subjects systems, methods and kits find use in a variety of different applications, several representative ones of which are described in detail as well. |
申请公布号 |
US8420395(B2) |
申请公布日期 |
2013.04.16 |
申请号 |
US20040003941 |
申请日期 |
2004.12.03 |
申请人 |
CALOS MICHELE;POETIC GENETICS, INC.;THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY |
发明人 |
CALOS MICHELE |
分类号 |
C12N15/63;A61K48/00;C07K14/505;C12N9/00;C12N9/22;C12N15/00;C12N15/11;C12N15/52;C12N15/82;C12N15/85;C12N15/86;C12N15/87;C12N15/90 |
主分类号 |
C12N15/63 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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