Cosmid Vector for Transforming Plant and Use Thereof

摘要

The present invention provides novel cosmid vectors for plant transformation. The cosmid vectors have a full length of 15 kb or less and contain: 1) an origin of replication of an IncP plasmid, but not any origin of replication of other plasmid groups; 2) the trfA1 gene of an IncP plasmid; 3) an oriT of an IncP plasmid; 4) the incC1 gene of an IncP plasmid; 5) a cos site of lambda phage, which is located outside the T-DNA; 6) a drug resistance gene expressed in E. coli and a bacterium of Agrobacterium; 7) a T-DNA right border sequence of a bacterium of Agrobacterium; 8) a T-DNA left border sequence of a bacterium of Agrobacterium; 9) a selectable marker gene for plant transformation located between 7) and 8) and expressed in a plant; and 10) restriction endonuclease recognition site(s) located between 7) and 8) for cloning a foreign gene.