KIT AND METHOD FOR DETECTING MUTATION OF PREDETERMINED SITE IN DNA SAMPLE AND USE THEREOF

摘要

Provided in the present invention are a primer, a kit and a method for detecting the mutation of a predetermined site in a DNA sample, and the use thereof. The method for detecting the mutation of a predetermined site in a DNA sample comprises the following steps: a PCR amplification reaction is carried out on the DNA samples using an amplification primer; an oligonucleotide extension reaction is carried out using an extension primer and ddNTP and taking the resulting amplification product as a template, so as to obtain an extension product with one base on the 3' terminus of the extension primer; and the mutation type of the predetermined site is determined based on the molecular weight of the extension product.