SOLVENT FOR FREEZING EQUINE SEMEN, PREPARATION METHOD AND USES

摘要

The present invention relates to a medium for freezing equine semen that comprises: a) a Hank's Buffered Salt Solution supplemented with HEPES, glucose, lactose, BSA and native phosphocaseinate from milk; b) egg yolk (0.5-4% v/v); c) HCON (CH3)2 (1-4% v/v); and d) glycerol (0.5-2% v/v). The invention also relates to the method for obtaining the medium of the invention, which comprises: i) breaking an egg and separating white from yoke; ii) centrifuging the yoke resulting from i) at a speed of 2-2500 rpm, for a period of 15-20 minutes; iii) adding the yoke resulting from step ii) at 0.5 to 4% v/v to the Hank's Buffered Salt Solution supplemented with HEPES, glucose, lactose, BSA and native phosphocaseinate from milk; iv) adding the solution resulting from iii), HCON (CH3)2 at 1 to 4% v/v, glycerol at 0.5 to 2% v/v; and v) homogenizing the solution resulting from iv).