TRUNCATED GLUCAGON-LIKE PEPTIDE-1 (sGLP-1) AND ITS PREPARING METHOD AND USE

摘要

Glucagon-like peptide-1 (GLP-1) analogs for promoting secretion of insulin, named as truncated glucagon-like peptide-1 (sGLP-1), is composed of 26 amino acid residues with the sequence of His-X1-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Val-Ser-Ser-Tyr-Leu-Glu-Gly-Gln-Ala-Ala-Lys-Glu-Phe-Ile-Ala-Trp-Leu. The sGLP-1 according to the present invention has marked merits compared with current GLP-1 analogs, as follows: a) stronger binding affinity to insulin cell receptor and enhanced capacity to stimulate insulin secretion; b) resistance to degradation of dipeptidyl peptidase because of the substitution of Gly or Ser for Ala at amino-acid position 2, resulting in longer in vivo half life and improved curative effect; c) lowering synthetic cost due to shorter sequence.