| 摘要 |
There is provided a microscope apparatus (100) including: a plurality of objective lenses (12) having different magnifications; an imaging system (7) that receives light, which is generated from a sample (8) and emitted from the objective lens (12) when excitation light (L2) is emitted to a sample including a fluorescent material that is activated when irradiated with activation light (L1) having a predetermined wavelength and fluoresces to be inactivated when irradiated with excitation light (L2) having a different wavelength from the activation light (L1) in the activation state and that images the light in a state where an astigmatic difference is given to the image of the sample (8); and an imaging device (14) that captures the image of the sample (8) from the imaging system (7). The imaging system (7) includes an astigmatic difference changing device (50) that changes the astigmatic difference according to the depth of focus of the objective lens (12). |
