发明名称 |
METHODS OF GENERATING ANTIGEN-SPECIFIC CD4+CD25+ REGULATORY T CELLS, COMPOSITIONS AND METHODS OF USE |
摘要 |
<p>The present invention provides methods for generating mammalian T cell populations comprising antigen-specific CD4+CD25+ regulatory T cells from freshly isolated CD4+CD25− T cells. The method comprises selecting CD4+CD25− T cells from a sample obtained from a mammalian subject; determining the MHC Class II type of the subject; inducing the generation of antigen-specific regulatory T cells by contacting the isolated CD4+CD25− T cells in a culture vessel with an induction agent for a time period sufficient to generate antigen-specific CD4+CD25+ regulatory T cells; and selecting the CD4+CD25+ antigen-specific regulatory T cells by sorting the cells in the induction culture with a selection agent comprising at least one artificial multimeric MHC Class II/peptide complex that corresponds to the MHC Class II type of the subject.</p> |
申请公布号 |
EP1812563(B1) |
申请公布日期 |
2013.04.10 |
申请号 |
EP20050825131 |
申请日期 |
2005.10.28 |
申请人 |
BENAROYA RESEARCH INSTITUTE AT VIRGINIA MASON |
发明人 |
BUCKNER, JANE, H.;WALKER, MINDI, R. |
分类号 |
C12N5/0783;A01N63/00;A61K39/00 |
主分类号 |
C12N5/0783 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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