The present invention relates to a method for the determination of a nucleic acid sequence by physical manipulation. In particular, the said method comprises the steps of denaturing a double-stranded nucleic acid molecule corresponding to the said nucleic acid sequence by applying a physical force to the said molecule; and detecting a blockage of the renaturation of the double-stranded nucleic acid molecule. More specifically, the method comprises the steps of denaturing a double-stranded nucleic acid molecule corresponding to the said nucleic acid sequence by applying a physical force to the said molecule; providing a single-stranded nucleic acid molecule; renaturing the said double stranded nucleic acid molecule in the presence of the said single-stranded nucleic acid molecule; and detecting a blockage of the renaturation of the double-stranded nucleic acid.
申请公布号
EP2576818(A1)
申请公布日期
2013.04.10
申请号
EP20110721549
申请日期
2011.05.26
申请人
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS;ECOLE NORMALE SUPERIEURE (ENS)
发明人
BENSIMON, DAVID;ALLEMAND, JEAN-FRANCOIS;MANOSAS, MARIA;DING, FANG-YUAN;CROQUETTE, VINCENT