发明名称 |
NOVEL CHELATOR AND USE THEREOF |
摘要 |
<p>The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity. The dimeric pentadentate chelator according to the invention has the following general formula: wherein Sc is a scaffold or a connecting structure that contains at least two functional groups enabling coupling of two pentadentate chelators (PD), and PD is a pentadentate chelator having the formula:</p> |
申请公布号 |
EP2576502(A1) |
申请公布日期 |
2013.04.10 |
申请号 |
EP20110790086 |
申请日期 |
2011.05.30 |
申请人 |
GE HEALTHCARE BIO-SCIENCES AB |
发明人 |
ALGOTSSON, MATTIAS;ANDERSSON, LARS, C.;BREKKAN, EGGERT;HEDEMYR, LEE;MALOISEL, JEAN-LUC;PALMGREN, RONNIE |
分类号 |
C07C237/10;B01D15/38;B01J20/22;B01J20/289;C07C323/60;C07K1/22 |
主分类号 |
C07C237/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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