| 摘要 |
PURPOSE: A method for preparing cysteine protein G-fixed magnetic silica nanoparticles is provided to improve sensitivity and to simplify the process by one-step. CONSTITUTION: A cysteine protein G-fixed magnetic silica nanoparticle is prepared by substituting chlorosilane onto the surface of silica-coated magnetic silica nanoparticles and conjugating cysteine protein G to a chloro group of the nanoparticles. The nanomagentic material is magnetite(Fe_3O_4), hematite(alpha-Fe_2O_3), maghemite(gamma-Fe_2O_3), nickel, cobalt, or compounds thereof. The cysteine protein G is a cysteine functional group-tagged protein G multimer. |
