发明名称 |
ASSAY FOR INHIBITORS OF CIP/KIP PROTEIN DEGRADATION |
摘要 |
An assay and system compatible with high throughput screening (HTS) that is capable of identifying inhibitors, such as small-molecule inhibitors, of the degradation of the Cdk inhibitor p21, are described. The assay is based on the use of fusion protein comprising (i) a p2 polypeptide; and (i) a reporter protein linked to the C-terminal of said p21 polypeptide, wherein the fusion protein has a half-life that is similar to that of the p21 polypeptide. Inhibitors identified by this assay may be useful to inhibit the proliferation of tumor cells, and thus for the treatment of cancers. |
申请公布号 |
WO2013044391(A1) |
申请公布日期 |
2013.04.04 |
申请号 |
WO2012CA50682 |
申请日期 |
2012.09.28 |
申请人 |
UNIVERSITE DE MONTREAL |
发明人 |
MARINIER, ANNE;MELOCHE, SYLVAIN;PELLETIER, BENOIT |
分类号 |
C12Q1/68;C12Q1/02;C40B30/06;G01N33/50;G01N33/52 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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