METHOD FOR PRODUCING POLYPEPTIDE FRAGMENT WITH HIGH EFFICIENCY, WHICH IS SUITABLE FOR NCL METHOD

摘要

[Problem] To provide a method for producing a polypeptide fragment with high efficiency, which is suitable for an NCL method. [Solution] A production method comprising: a step of reacting a polypeptide which comprises a first polypeptide fragment having a cysteine residue as an N-terminal thereof and a second polypeptide fragment with CNBr to produce the first polypeptide fragment having a cysteine residue as an N-terminal thereof and a third polypeptide fragment, wherein the first polypeptide fragment and the second polypeptide fragment are linked to each other through an intervening sequence -Cys-W-(His)n-Z-Met-; and a step of reacting the third polypeptide fragment with a compound represented by formula (I) and a compound represented by formula (II) sequentially to produce the second polypeptide fragment having a modified C-terminal.