发明名称 |
MEASUREMENT OF LACTIC ACID IN BIOLOGICAL FLUIDS |
摘要 |
We propose a procedure for the determination of lactic acid or lactate in biological fluids, which is simple and low cost with respect to the known methodologies. This procedure can be used by unskilled personnel and it can be implemented in disposable test strips and portable measure devices. The procedure is based on the photochemical reaction of lactic acid with Fe(III), which is reduced to Fe(II) when irradiated with UV light. The Fe(II), produced proportionally to the amount of lactate in the sample, is determined electrochemically or using its reaction with a colored complexing agent, such as 1,10- phenanthroline, and determined by spectrophotometry. This method is advantageous both for the low cost of the reagents employed, and for their short and long term stability. |
申请公布号 |
WO2013045443(A1) |
申请公布日期 |
2013.04.04 |
申请号 |
WO2012EP68867 |
申请日期 |
2012.09.25 |
申请人 |
BRAMANTI, EMILIA;ZUCCHINI, FABRIZIO;ONOR, MASSIMO;DI MURO, VINCENZO |
发明人 |
BRAMANTI, EMILIA;ZUCCHINI, FABRIZIO;ONOR, MASSIMO;DI MURO, VINCENZO |
分类号 |
G01N21/84;G01N21/64;G01N21/76;G01N21/78;G01N27/327;G01N33/487 |
主分类号 |
G01N21/84 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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