发明名称 |
CONTINUOUS EXTENSION AND DEBLOCKING IN REACTIONS FOR NUCLLEIC ACIDS SYNTHESIS AND SEQUENCING |
摘要 |
A reaction mixture including (a) a nucleic acid having a primer hybridized to a template, (b) nucleotide analogs, wherein the nucleotide analogs have a blocking moiety; (c) a polymerase that is capable of forming an extended primer by adding the nucleotide analogs to the primer, and (d) a deblocking agent that is capable of removing the blocking moiety from the extended primer. Also provided is a method of synthesizing a polynucleotide including sequentially adding a plurality of the different nucleotides analogs to the nucleic acid via several reaction cycles in the reaction mixture, wherein each reaction cycle includes (i) the polymerase adding a nucleotide analog to the nucleic acid to form a transient nucleic acid species comprising a blocking moiety, and (ii) the deblocking agent modifying the transient nucleic acid species to remove the blocking moiety.
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申请公布号 |
US2013085073(A1) |
申请公布日期 |
2013.04.04 |
申请号 |
US201213627134 |
申请日期 |
2012.09.26 |
申请人 |
ILLUMINA CAMBRIDGE LIMITED;ILLUMINA CAMBRIDGE LIMITED |
发明人 |
MEULEMAN WOUTER;CRESSINA ELENA;SMITH GEOFFREY PAUL;JACKSON ROSAMOND;LIU XIAOHAI |
分类号 |
C40B20/00;C12P19/34;C12Q1/68;G01N21/64 |
主分类号 |
C40B20/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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