LINEAR AMPLIFICATION METHOD FOR TRACE DNA FRAGMENT

摘要

The amplification by an LM-PCR method has such a problem that amplification accuracy is not satisfactory although amplification sensitivity is high, and the amplification by a T7-IVT method has such a problem that amplification sensitivity is not satisfactory although amplification accuracy is high. The present invention provides a nucleic acid amplification method whereby it becomes possible to solve the problems of these conventional methods. A nucleic acid amplification method comprising the following step (a) and/or step (b): (a) a step of carrying out an enzymatic reaction of a terminal nucleotide transferase in the presence of DNA which cannot be added with a base at the 3'-terminal thereof with the terminal nucleotide transferase; and (b) a step of carrying out the enzymatic reaction of a Klenow enzyme in the co-presence of a poly(A-T7) oligo-DNA primer and the Klenow enzyme and in the presence of DNA that cannot undergo the formation of any double strand in a T7 region of the poly(A-T7) oligo-DNA primer.