发明名称 |
FILLER FOR AFFINITY CHROMATOGRAPHY AND METHOD FOR ISOLATING IMMUNOGLOBULIN |
摘要 |
<p>Provided are a filler for affinity chromatography which has excellent alkali resistance, and a method for isolating immunoglobulin. The filler for affinity chromatography is a filler in which a protein represented by the following formula (1) is immobilized on a carrier.
€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒR-R 2 €ƒ€ƒ€ƒ€ƒ€ƒ(1)
wherein R represents an amino acid sequence consisting of 4 to 300 amino acid residues containing a region consisting of 4 to 20 contiguous histidine residues; and R 2 represents an amino acid sequence capable of binding to immunoglobulin, the amino acid sequence consisting of 50 to 500 amino acid residues containing Z domain of Protein A or a fragment thereof, or a variant thereof, provided that the R binds to C-terminus or N-terminus of the R 2 .</p> |
申请公布号 |
EP2574631(A1) |
申请公布日期 |
2013.04.03 |
申请号 |
EP20110759404 |
申请日期 |
2011.03.23 |
申请人 |
JSR CORPORATION |
发明人 |
TAMORI, KOUJI;FUKUTA, TETSUO;MIYAJI, MASAAKI;WANG, YONG;ABE, TAKAYOSHI;OKANO, YUSUKE;MOMIYAMA, MASAKI;KAWAI, TAKAHIRO |
分类号 |
C07K17/08;B01J20/32;C07K1/22;C07K19/00;C12N15/09;G01N30/88 |
主分类号 |
C07K17/08 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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